Reporter

Part:BBa_K1423007:Design

Designed by: Kayla DeSanty   Group: iGEM14_WPI-Worcester   (2014-09-23)


Constitutive Banana Odor Generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 163
    Illegal BamHI site found at 1490
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 590
    Illegal SapI.rc site found at 1598


Design Notes

We ordered oligos for the constitutive promoter to assure that it would ligate into the Biobrick properly.


Source

The constitutive promoter and ribosome binding site, ATF1 gene, and double terminator composite part are both biobricks.

References